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Sample GSM635226 Query DataSets for GSM635226
Status Public on Jan 01, 2012
Title KLA 6hrs HBD3 rep2
Sample type RNA
 
Source name Bone marrow derived macrophages
Organism Mus musculus
Characteristics strain: Balb/c
cell type: Bone marrow derived macrophages
treatment: KLA 50ng/ml
time: 6 hours
Treatment protocol Macrophages at a concentration 5 x 10E5 cells per well (6 well plate) were incubated overnight in the absence of M-CSF. Cells were treated as described above, then incubated for 6 hour at 37 degrees.
Growth protocol Bone marrow was flushed from femurs of Balb/c mice and incubated for 7-10 days in the presence of macrophage colony stimulating factor (M-CSF, 20ng/ml)
Extracted molecule total RNA
Extraction protocol RNA bee (AMS Biotechnology)
Label biotin
Label protocol Amplified, biotinylated cRNA was synthesized using Illumina® TotalPrep Amplification Kit (Ambion, Austin, Texas)
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description KLA 6hrs HBD3 rep2
Data processing The data were normalised using quantile normalisation using LIMMA in R
 
Submission date Dec 06, 2010
Last update date Jan 01, 2012
Contact name Graeme Richard Grimes
E-mail(s) graeme.grimes@ed.ac.uk
Organization name University of Edinburgh, The Institute of Genetics and Cancer
Department Human Genetics Unit
Lab Bioinformatics Analysis Core
Street address Western General Hospital, Crewe Road
City Edinburgh
ZIP/Postal code EH4 2XU
Country United Kingdom
 
Platform ID GPL6887
Series (2)
GSE25871 Human Beta-Defensin 3 attenuates the pro-inflammatory effects of KDO2-Lipid A (KLA) at 6 hour
GSE25873 Effect of hBD3 on transcription in TLR4-stimulated macrophages

Data table header descriptions
ID_REF
VALUE quantile normalized signal

Data table
ID_REF VALUE
ILMN_2417611 50.2013175
ILMN_2762289 63.95753917
ILMN_2896528 647.4049417
ILMN_2721178 249.5749333
ILMN_2458837 55.28923833
ILMN_3033922 407.9275417
ILMN_3092673 982.2152
ILMN_1230777 669.2690667
ILMN_1246069 848.90255
ILMN_1232042 54.50171833
ILMN_1243193 646.15515
ILMN_2524361 55.45271417
ILMN_1242440 48.40478167
ILMN_1233188 50.23235333
ILMN_2543688 52.76153333
ILMN_1259789 54.6177025
ILMN_2816356 55.877555
ILMN_1224596 75.33825583
ILMN_1233643 54.65565083
ILMN_2808939 373.0978167

Total number of rows: 45281

Table truncated, full table size 1080 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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