|
| Status |
Public on Jul 22, 2022 |
| Title |
Control ChIP-seq from K562 (ENCLB457NMG) |
| Sample type |
SRA |
| |
|
| Source name |
Homo sapiens K562 cell line
|
| Organism |
Homo sapiens |
| Characteristics |
line: K562
biomaterial_type: cell line
description: K562 cell line used as an untransfected matched control to one stably transfected with a construct containing an eGFP-tagged transcription factor.
biosample encode accession: ENCBS209TSR (SAMN29769685)
age: 53 year
Sex: female
health state: chronic myelogenous leukemia (CML)
dev stage: adult
donor_id: ENCDO000AAD
lab: Kevin White, UChicago
source: Kevin White
link: ENCBS209TSR at ENCODE; https://www.encodeproject.org/ENCBS209TSR/
link: Derived from ENCODE donor ENCDO000AAD; https://www.encodeproject.org/ENCDO000AAD/
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
general protocol: https://www.encodeproject.org/documents/43a5968c-9732-4d63-b35c-78ca16749e92/@@download/attachment/wgEncodeUchicagoTfbs.release2.html.pdf
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina Genome Analyzer II |
| |
|
| Description |
https://www.encodeproject.org/experiments/ENCSR828WYU/
***************
biological replicate number: 1
technical replicate number: 1
description: Control ChIP-seq matched with ChIP-seq on human K562 eGFP-tagged transcription factors. The White lab used goat anti-GFP antibody to perform ChIP in untagged K562 cells as a background control.
experiment encode accession: ENCSR828WYU
assay title: Control ChIP-seq
assembly: GRCh38, hg19
encode release date: 2011-06-03
lab: Kevin White, UChicago
library encode accession: ENCLB457NMG
fragmentation methods: see document
|
| Data processing |
See GSM*_README.txt supplementary file linked below
|
| |
|
| Submission date |
Jul 22, 2022 |
| Last update date |
Jul 24, 2022 |
| Contact name |
ENCODE DCC |
| E-mail(s) |
encode-help@lists.stanford.edu
|
| Organization name |
ENCODE DCC
|
| Street address |
300 Pasteur Dr
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305-5120 |
| Country |
USA |
| |
|
| Platform ID |
GPL9115 |
| Series (1) |
| GSE209294 |
Control ChIP-seq from K562 (ENCSR828WYU) |
|
| Relations |
| BioSample |
SAMN29769685 |
| SRA |
SRX16499908 |
