tissue: Vastus lateralis muscle age: Young gender: Male
Extracted molecule
total RNA
Extraction protocol
Homogenize the muscle tissue in TRI reagent and extracted RNA according to manufacturer's protocol, and then purified using Rneasy Micro Kit (Qiagen).
Label
biotin
Label protocol
40ng of total RNA was labeled using NuGen WT-Ovation RNA Amp v2 kit and protocol
Hybridization protocol
Affymetrix Fluidics Station 400
Scan protocol
Affymetrix GeneChip Scanner 3000 7G
Description
GPL750
Data processing
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1500.
