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Sample GSM637533 Query DataSets for GSM637533
Status Public on Feb 21, 2012
Title Muscle_Young_Men_1.0
Sample type RNA
 
Source name Vastus lateralis muscle
Organism Homo sapiens
Characteristics tissue: Vastus lateralis muscle
age: Young
gender: Male
Extracted molecule total RNA
Extraction protocol Homogenize the muscle tissue in TRI reagent and extracted RNA according to manufacturer's protocol, and then purified using Rneasy Micro Kit (Qiagen).
Label biotin
Label protocol 40ng of total RNA was labeled using NuGen WT-Ovation RNA Amp v2 kit and protocol
 
Hybridization protocol Affymetrix Fluidics Station 400
Scan protocol Affymetrix GeneChip Scanner 3000 7G
Description GPL750
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1500.
 
Submission date Dec 08, 2010
Last update date Feb 21, 2012
Contact name Shawn T. Estrem
Organization name Eli Lilly and Company
Street address Lilly Corporate Center
City Indianapolis
ZIP/Postal code 46285
Country USA
 
Platform ID GPL570
Series (1)
GSE25941 Effects of age on the skeletal muscle transcriptome

Data table header descriptions
ID_REF
VALUE Signal
ABS_CALL indicating whether the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 4877.69 P 4.42873e-05
AFFX-BioB-M_at 9325.14 P 4.42873e-05
AFFX-BioB-3_at 7273.57 P 4.42873e-05
AFFX-BioC-5_at 15885.5 P 4.42873e-05
AFFX-BioC-3_at 11455.4 P 4.42873e-05
AFFX-BioDn-5_at 32803.4 P 4.42873e-05
AFFX-BioDn-3_at 43222.4 P 4.42873e-05
AFFX-CreX-5_at 82370.4 P 5.16732e-05
AFFX-CreX-3_at 80748.1 P 4.42873e-05
AFFX-DapX-5_at 39.0447 A 0.175328
AFFX-DapX-M_at 2.23941 A 0.998407
AFFX-DapX-3_at 24.5162 A 0.772364
AFFX-LysX-5_at 4.84005 A 0.883887
AFFX-LysX-M_at 13.013 A 0.699394
AFFX-LysX-3_at 3.35449 A 0.971543
AFFX-PheX-5_at 9.14483 A 0.760937
AFFX-PheX-M_at 6.54497 A 0.891021
AFFX-PheX-3_at 35.0646 A 0.425956
AFFX-ThrX-5_at 46.0757 A 0.313723
AFFX-ThrX-M_at 5.20414 A 0.937071

Total number of rows: 54675

Table truncated, full table size 1662 Kbytes.




Supplementary file Size Download File type/resource
GSM637533.CEL.gz 5.2 Mb (ftp)(http) CEL
GSM637533.CHP.gz 292.5 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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