|
| Status |
Public on Sep 16, 2022 |
| Title |
Case 7 YCC-T |
| Sample type |
RNA |
| |
|
| Source name |
Primary hepatobiliary tumor
|
| Organism |
Homo sapiens |
| Characteristics |
histology: Cholangiocarcinoma (less than 40 generations), tumor part
age: 39
Sex: M
molecule: miRNA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted and purified each from 5 ul samples of tissue (or serum exosome) using an miRNeasy mini kit (Qiagen, Inc.).
|
| Label |
Cy5
|
| Label protocol |
microRNA was labeled using 3D-Gene® miRNA Labeling kit (Toray Industries, Inc.) in accordance with the manufacturer's instructions.
|
| |
|
| Hybridization protocol |
Hybridized for 16 h at 32 ºC with rotary shaker (250 rpm). Hybridization buffer and washing protocol was attached in the 3D-Gene® miRNA oligo chip kit.
|
| Scan protocol |
3D-Gene® Scanner (Toray Industries, Inc.) was used for scanning. Images were quantified using Extraction version 2.0.0.6 (Toray Industries, Inc.).
|
| Data processing |
Fluorescent value excited at 635 nm was degitalized as the data.
Each fluorescent raw value was run in batches from standardization (in the sample direction by Z-score) to principal component analysis.
|
| |
|
| Submission date |
Jul 24, 2022 |
| Last update date |
Sep 16, 2022 |
| Contact name |
Satoko Takizawa |
| E-mail(s) |
satoko.takizawa.f3@mail.toray
|
| Phone |
+81-467-32-9351
|
| Organization name |
Toray Industries, Inc.
|
| Street address |
6-10-1, Tebiro
|
| City |
Kanagawa |
| State/province |
Kamakura |
| ZIP/Postal code |
2480036 |
| Country |
Japan |
| |
|
| Platform ID |
GPL21263 |
| Series (2) |
| GSE209611 |
MicroRNA and mRNA expression profiles of hepatobiliary tumor patients' tissue together with those of non-tumor part tissues and serum exosomes. (miRNA) |
| GSE209875 |
MicroRNA and mRNA expression profiles of hepatobiliary tumor patients' tissue together with those of non-tumor part tissues and serum exosomes. |
|
