|
| Status |
Public on Jun 30, 2023 |
| Title |
14KALS, iPSC-derived motor neurons from patient with ALS, non, 60h |
| Sample type |
SRA |
| |
|
| Source name |
ALS05
|
| Organism |
Homo sapiens |
| Characteristics |
genotype: WT
treatment: non
time: 60h
cell line: 14KALS
cell type: iPSC-derived motor neurons from patient with ALS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested using Rneasy Micro Kit (Qiagen). 0.2 ng of total RNA was used for the construction of sequencing libraries.
RNA libraries for RNA-seq were prepared using Nextera XT DNA Library Prep Kit following manufacturer's protocols.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
SMART-seq
|
| Data processing |
Quality Control of the sequences was performed by fastp (version 0.23.2).
Read counts were calculated by salmon (version 1.7.0), and the read count data outputs by salmon were processed by tximport (version 1.22.0).
The processed data were subjected to Transcripts Per Million (TPM) normalization among groups.
|
| |
|
| Submission date |
Jul 25, 2022 |
| Last update date |
Jun 14, 2024 |
| Contact name |
Satoru Morimoto |
| Organization name |
Keio University
|
| Department |
Keio Regenerative Medicine Research Center (KRM)
|
| Lab |
Okano lab
|
| Street address |
3－25－10, Tonomachi, Kawasaki-ku, Kawasaki-shi
|
| City |
Kanagawa |
| ZIP/Postal code |
2100821 |
| Country |
Japan |
| |
|
| Platform ID |
GPL24676 |
| Series (1) |
| GSE209696 |
Ropinirole hydrochloride identified by iPSC drug discovery for amyotrophic lateral sclerosis: a single-centered, randomized, double-blind, placebo-controlled phase 1/2a trial |
|
| Relations |
| BioSample |
SAMN29944170 |
| SRA |
SRX17305929 |
