|
| Status |
Public on May 31, 2023 |
| Title |
22RV1_COM_rep3 |
| Sample type |
SRA |
| |
|
| Source name |
22RV1
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: 22RV1
treatment: 0.03mg/kg ORY-1001(3 times/week), 32mg/kg I-BET762 (6 times/week)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Rneasy Mini Kit (QIAGEN) following manufacturer’s protocol.
RNA-seq libraries were performed by using TruSeq® Strnd Total RNA LT (Illumina).
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
NextSeq 2000 |
| |
|
| Description |
Xenograft_22RV1_ORYIBETCOM_samples_count_table.txt.gz
|
| Data processing |
The sequence quality of raw reads was checked by FastQC (version 0.11.9).
Raw reads were aligned to the human reference genome (hg19) using STAR (version 2.7.2) followed by counting with featureCounts (version 1.6.4) from GRCh37 Ensembl reference.
Assembly: hg19
Supplementary files format and content: txt file for raw counts of sequence reads
|
| |
|
| Submission date |
Jul 27, 2022 |
| Last update date |
May 31, 2023 |
| Contact name |
Changmeng Cai |
| E-mail(s) |
changmeng.cai@umb.edu
|
| Organization name |
University of Massachusetts Boston
|
| Street address |
100 William T Morrissey Blvd
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02125 |
| Country |
USA |
| |
|
| Platform ID |
GPL30173 |
| Series (2) |
| GSE209887 |
LSD1 inhibition disrupts super-enhancer activities in CRPC [RNA-seq] |
| GSE209889 |
LSD1 inhibition disrupts super-enhancer activities in CRPC |
|
| Relations |
| BioSample |
SAMN29996320 |
| SRA |
SRX16690901 |
