|
| Status |
Public on Feb 16, 2011 |
| Title |
untreated purified beta cells |
| Sample type |
RNA |
| |
|
| Source name |
purified beta cells, NOD.RAG-/- strain, untreated
|
| Organism |
Mus musculus |
| Characteristics |
genotype/variation: RAG-/-
strain: NOD
organ: Pancreas
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Ambion RNAqeuous Micro Kit
|
| Label |
Biotin
|
| Label protocol |
Affymetrix® GeneChip® WT Sense Target Labeling
|
| |
|
| Hybridization protocol |
Affymetrix GeneChip® Fluidics Station 450
|
| Scan protocol |
Affymetrix GeneChip® Scanner 3000 7G, GCOS
|
| Data processing |
GCOS, Expression Console, and DNAStar Arraystar 4.0
affymetrix-algorithm-name = RMA
affymetrix-algorithm-version = 1.0
affymetrix-algorithm-param-Background correction method = RMA Background Correction
affymetrix-algorithm-param-Normalization method = Quantile
affymetrix-algorithm-param-Summarization method = Median polish
|
| |
|
| Submission date |
Dec 17, 2010 |
| Last update date |
Feb 16, 2011 |
| Contact name |
Emil Unanue |
| E-mail(s) |
unanue@pathology.wustl.edu
|
| Organization name |
Washington University School of Medicine
|
| Department |
Pathology and Immunology
|
| Street address |
660 S Euclid Ave Box 8118
|
| City |
Saint Louis |
| State/province |
MO |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE26147 |
Examination of inflammatory transcripts during a transfer model of type I diabetes. |
|
