|
| Status |
Public on May 24, 2023 |
| Title |
Flcn_KO cDC1, 3 |
| Sample type |
RNA |
| |
|
| Source name |
splenic cDC1 were sorted from Flcn∆DC mice
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
cell type: splenic cDC1
genotype: Flcn_KO
|
| Treatment protocol |
Sorted cells were lysed with RLT lysis buffer of Rneasy Micro kit from Qiagen.
|
| Growth protocol |
Spleens were digested, and denritic cells were enriched with anti-CD11c MicroBeads prior to sorting. Splenic cDC1 (CD11c+CD8α+CD24+TCRβ−CD49b−B220−) were sorted from naïve WT and Flcn∆DC mice.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA samples were prepared according to the prototol of Rneasy Micro kit from Qiagen,
|
| Label |
biotin
|
| Label protocol |
High-quality RNA was processed using Thermo Fisher (Affymetrix) Whole Transcript (WT) Plus assay kit
|
| |
|
| Hybridization protocol |
Labeled cDNA was included in a hybridization mix that was incubated on the Clariom S mouse array for 16 hours at 45◦c while rotating at 60 rpm.
|
| Scan protocol |
GeneChips were scanned using the AffymetrixGeneChip Scanner 3000 7G.
|
| Data processing |
Probe signals were quantile normalized and summarized by the RMA algorithm using the Affymetrix Expression Console software v1.1
|
| |
|
| Submission date |
Jul 31, 2022 |
| Last update date |
May 24, 2023 |
| Contact name |
Hongbo Chi |
| E-mail(s) |
hongbo.chi@stjude.org
|
| Organization name |
St Jude Children's Research Hospital
|
| Department |
Immunology
|
| Street address |
262 Danny Thomas Place
|
| City |
Memphis |
| State/province |
TN |
| ZIP/Postal code |
38105 |
| Country |
USA |
| |
|
| Platform ID |
GPL23038 |
| Series (2) |
| GSE210148 |
SLC38A2 and glutamine signaling in cDC1 dictate anti-tumor immunity [array] |
| GSE210155 |
SLC38A2 and glutamine signaling in cDC1 dictate anti-tumor immunity |
|
