|
| Status |
Public on Dec 22, 2010 |
| Title |
Tbx5+/- Brg1 +/- rep3 |
| Sample type |
RNA |
| |
|
| Source name |
mouse E11.5 hearts
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57Bl/6/BLKSW mix
genotype/variation: Tbx5+/- Brg1 +/-
tissue: heart
developmental stage: E11.5
|
| Growth protocol |
Embryonic hearts were dissected at E11.5
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy Micro (December 2007)
|
| Label |
biotin
|
| Label protocol |
Standard methods for biotin labeling were used.
|
| |
|
| Hybridization protocol |
Affymetrix mouse Gene ST 1.0 arrays were hybridized and scanned according to manufacturer recommendations.
|
| Scan protocol |
Affymetrix mouse Gene ST 1.0 arrays were hybridized and scanned according to manufacturer recommendations.
|
| Data processing |
Raw intensities from CEL files were analyzed using Affymetrix Power Tools (APT, version 1.10.1) to generate RMA (robust multi-array average; 49) expression intensities on a log2 scale for each probe set
|
| |
|
| Submission date |
Dec 20, 2010 |
| Last update date |
Dec 22, 2010 |
| Contact name |
Alisha Holloway |
| E-mail(s) |
alisha.holloway@gladstone.ucsf.edu
|
| Organization name |
Gladstone Institutes
|
| Street address |
1650 Owens Street
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94158 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE26191 |
Chromatin Remodeling Complex Dosage Modulates Transcription Factor Function in Heart Development |
|
