|
Status |
Public on Dec 22, 2010 |
Title |
wild type rep1 |
Sample type |
RNA |
|
|
Source name |
mouse E11.5 hearts
|
Organism |
Mus musculus |
Characteristics |
strain: C57Bl/6/BLKSW mix genotype/variation: wild type tissue: heart developmental stage: E11.5
|
Growth protocol |
Embryonic hearts were dissected at E11.5
|
Extracted molecule |
total RNA |
Extraction protocol |
Qiagen RNeasy Micro (December 2007)
|
Label |
biotin
|
Label protocol |
Standard methods for biotin labeling were used.
|
|
|
Hybridization protocol |
Affymetrix mouse Gene ST 1.0 arrays were hybridized and scanned according to manufacturer recommendations.
|
Scan protocol |
Affymetrix mouse Gene ST 1.0 arrays were hybridized and scanned according to manufacturer recommendations.
|
Data processing |
Raw intensities from CEL files were analyzed using Affymetrix Power Tools (APT, version 1.10.1) to generate RMA (robust multi-array average; 49) expression intensities on a log2 scale for each probe set
|
|
|
Submission date |
Dec 20, 2010 |
Last update date |
Dec 22, 2010 |
Contact name |
Alisha Holloway |
E-mail(s) |
alisha.holloway@gladstone.ucsf.edu
|
Organization name |
Gladstone Institutes
|
Street address |
1650 Owens Street
|
City |
San Francisco |
State/province |
CA |
ZIP/Postal code |
94158 |
Country |
USA |
|
|
Platform ID |
GPL6246 |
Series (1) |
GSE26191 |
Chromatin Remodeling Complex Dosage Modulates Transcription Factor Function in Heart Development |
|