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| Status |
Public on Nov 09, 2022 |
| Title |
mammay epithelial cells,Ealy lactation IP,E2 |
| Sample type |
SRA |
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| Source name |
mammary gland
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| Organism |
Capra hircus |
| Characteristics |
tissue: mammary gland
cell type: mammary epithelial cells
treatment: m6A
breed: Laoshan
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| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using a TRIzol kit (Invitrogen, USA).
Eukaryotic mRNA from the extracted total RNA was enriched using Oligo (dT) beads, and a Ribo-ZeroTM Magnetic Kit (Epicentre, USA) was used to remove rRNA and enrich prokaryotic mRNA. Then, the enriched mRNA fragments were divided into short fragments using fragment buffer, and the RNA was divided into 2 samples, 1 of which was used as the input control. The transcriptome sequencing library was constructed to eliminate noise during the capture of methylated fragments, another RNA sample was enriched with an m6A-specific antibody.
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| Library strategy |
OTHER |
| Library source |
transcriptomic |
| Library selection |
other |
| Instrument model |
Illumina NovaSeq 6000 |
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| Data processing |
fastp (version 0.18.0)
reads containing adaptors were removed;
reads containing more than 10% unknown nucleotides (N) were removed;
reads containing more than 50% of low-quality bases (q value ≤ 20) were removed
Library strategy: MeRIP-Seq
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| Submission date |
Aug 03, 2022 |
| Last update date |
Nov 09, 2022 |
| Contact name |
君 舒 王 |
| E-mail(s) |
yiyi64340@gmail.com
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| Phone |
17866849101
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| Organization name |
山东农业大学
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| Department |
动物科技学院
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| Lab |
动物保护生物学实验室
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| Street address |
泰前街道
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| City |
泰安 |
| State/province |
山东省 |
| ZIP/Postal code |
271000 |
| Country |
China |
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| Platform ID |
GPL27106 |
| Series (1) |
| GSE210386 |
Identification and functional analysis of m6A in the mammary gland tissues of dairy goats at the early and peak lactation stages |
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| Relations |
| BioSample |
SAMN30118357 |
| SRA |
SRX16801786 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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