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Sample GSM6429547

Query DataSets for GSM6429547

Status

Public on Nov 09, 2022

Title

mammary epithelial cells,Early lactation,E1

Sample type

SRA

Source name

mammary gland

Organism

Capra hircus

Characteristics

tissue: mammary gland
cell type: mammary epithelial cells
breed: Laoshan

Extracted molecule

total RNA

Extraction protocol

Total RNA was extracted using a TRIzol kit (Invitrogen, USA).
Eukaryotic mRNA from the extracted total RNA was enriched using Oligo (dT) beads, and a Ribo-ZeroTM Magnetic Kit (Epicentre, USA) was used to remove rRNA and enrich prokaryotic mRNA. Then, the enriched mRNA fragments were divided into short fragments using fragment buffer, and the RNA was divided into 2 samples, 1 of which was used as the input control. The transcriptome sequencing library was constructed to eliminate noise during the capture of methylated fragments, another RNA sample was enriched with an m6A-specific antibody.

Library strategy

RNA-Seq

Library source

transcriptomic

Library selection

cDNA

Instrument model

Illumina NovaSeq 6000

Data processing

fastp (version 0.18.0)
reads containing adaptors were removed;
reads containing more than 10% unknown nucleotides (N) were removed;
reads containing more than 50% of low-quality bases (q value ≤ 20) were removed

Submission date

Aug 03, 2022

Last update date

Nov 09, 2022

Contact name

君舒王

E-mail(s)

yiyi64340@gmail.com

Phone

17866849101

Organization name

山东农业大学