|
| Status |
Public on Aug 06, 2022 |
| Title |
model 2 at 6H,brain |
| Sample type |
RNA |
| |
|
| Source name |
model 2 at 6H
|
| Organism |
Rattus |
| Characteristics |
strain: Sprague Dawley
tissue: brain
group: model 2
|
| Treatment protocol |
The rats were randomly divided into 3 groups according to a uniform design (n=3/group): 6h sham group, 6h vehicle control group, 6h YJ groups (25mg•kg−1). The sham operation group was subjected to only preoperative anesthesia and blood vessel separation; the other groups were subjected to the MCAO model. The animals were sacrificed to obtain brain, spleen and blood respectively. Furthermore, brains and spleens were made as homogenate and white blood cells were separated from whole blood for further tests.
|
| Growth protocol |
All animals were housed individually at 22 ± 2 °C with a relative humidity of 50 ± 10% and a 12-h light/12-h dark cycle.The animals were kept in a pathogen-free environment with free access to food and water.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction of total RNA was performed according to the manufacturer's instructions of Qiagen RNA kit.
|
| Label |
biotin
|
| Label protocol |
M-MLV reverse transcription (Promega) was used to synthesize cDNA.
|
| |
|
| Hybridization protocol |
Total RNA was reverse transcribed, amplified, labelled and hybridized to Rat Genome 1.0 arrays (Affymetrix).
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000 7G.
|
| Description |
M2-6H-NAO
|
| Data processing |
The data were analyzed with RMA using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Aug 04, 2022 |
| Last update date |
Aug 06, 2022 |
| Contact name |
Liming Liu |
| E-mail(s) |
liuliming2022@126.com
|
| Phone |
18813067753
|
| Organization name |
Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences
|
| Street address |
16 Dongzhimen nei south street, Dongcheng District, Beijing, China
|
| City |
Beijing |
| ZIP/Postal code |
100700 |
| Country |
China |
| |
|
| Platform ID |
GPL6247 |
| Series (1) |
| GSE210512 |
The network Regulation of Yiqi Jiedu Formulae against Cerebral Ischemia Based on Central-periphery Inflammation System |
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