|
| Status |
Public on Aug 14, 2022 |
| Title |
Infected fish 10, day 23, head kidney |
| Sample type |
RNA |
| |
|
| Source name |
Head kidney
|
| Organism |
Salmo salar |
| Characteristics |
agent: Moritella viscosa
time point: day 23
|
| Treatment protocol |
Fish were batch challenged with M. viscosa. The challenge study was conducted over the course of 34 days, with temperatures ranging from 8-9.5°C throughout the period. Mortality was recorded on a daily basis, and cause of death was confirmed by bacteriological sampling. Ten fish were collected from controls and challenge tanks at 9, 23, 34 days post-challenge (referred to as time points 1, 2, and 3 respectively). Additionally, 10 control fish were taken from the challenge tank prior to introduction of the bacterial culture. For each fish, samples were taken of the head-kidney (HK), as well as from the site of external ulcers, or from the abdomen if the fish did not exhibit visible lesions despite being infected
|
| Growth protocol |
Salmon post smolts were kept in tanks with sea water
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the RNeasy® Mini Kit (Qiagen)
|
| Label |
Cy3
|
| Label protocol |
Amplified and labeled with Low Input Quick Amp Labeling Kit (Agilent).
|
| |
|
| Hybridization protocol |
Hybridized according to Agilent One-Color Microarray-Based Gene Expression Analysis protocol
|
| Scan protocol |
Arrays were scanned with Agilent G2565BA DNA microarray scanner at a resolution of 5 microns
|
| Data processing |
Images were processed using the standard procedures of the Agilent Feature Extraction (FE) Software version 9.5.1. After removal of flagged spots lowess normalization was performed
|
| |
|
| Submission date |
Aug 11, 2022 |
| Last update date |
Aug 14, 2022 |
| Contact name |
Aleksei Krasnov |
| E-mail(s) |
aleksei.krasnov@nofima.no
|
| Phone |
+47 97602165
|
| Organization name |
Nofima AS
|
| Department |
Fish Health
|
| Street address |
Osloveien 1
|
| City |
Aas |
| ZIP/Postal code |
1430 |
| Country |
Norway |
| |
|
| Platform ID |
GPL28080 |
| Series (1) |
| GSE211004 |
Differential expression of miRNAs and mRNAs in Moritella viscosa challenge Atlantic salmon |
|
