|
| Status |
Public on Dec 08, 2011 |
| Title |
Homo_sapiens_genome_rep2_E |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
FoxA1 ChIP DNA (abl)
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: abl
antibody: FoxA1
phenotype: androgen-independent
antibody manufacturer: Abcam
antibody catalog #: ab23738
|
| Growth protocol |
LNCaP and abl were cultured in RPMI 1640/10% FBS and RPMI 1640/10% charcoal-stripped FBS, respectively.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
FoxA1 ChIP was performed followed by amplification using ligation-mediated PCR.
|
| Label |
biotin
|
| Label protocol |
The amplified ChIP'ed DNA was labelled with biotin.
|
| |
|
| Channel 2 |
| Source name |
FoxA1 ChIP DNA (LNCaP)
|
| Organism |
Homo sapiens |
| Characteristics |
phenotype: androgen-dependent
cell line: LNCaP
antibody: FoxA1
antibody manufacturer: Abcam
antibody catalog #: ab23738
|
| Growth protocol |
LNCaP and abl were cultured in RPMI 1640/10% FBS and RPMI 1640/10% charcoal-stripped FBS, respectively.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
FoxA1 ChIP was performed followed by amplification using ligation-mediated PCR.
|
| Label |
biotin
|
| Label protocol |
The amplified ChIP'ed DNA was labelled with biotin.
|
| |
|
| |
| Hybridization protocol |
standard Affymetrix protocol
|
| Scan protocol |
standard Affymetrix protocol
|
| Description |
replicate-2 chip-E
|
| Data processing |
Supplementary files contain results from Model Based analysis of tiling arrays (MAT) was used to process the data as described previously: Johnson, et al, 2006, PMID: 16895995
|
| |
|
| Submission date |
Dec 28, 2010 |
| Last update date |
Jun 25, 2013 |
| Contact name |
LIGUO WANG |
| E-mail(s) |
wang.liguo@mayo.edu
|
| Organization name |
Mayo Clinic
|
| Department |
Division of Computational Biology
|
| Street address |
200 1st St SW
|
| City |
Rochester |
| State/province |
MN |
| ZIP/Postal code |
55905 |
| Country |
USA |
| |
|
| Platform ID |
GPL4914 |
| Series (1) |
| GSE26329 |
MYBL2/CREB1-Dependent Distinct FoxA1 Cistrome Directs G1/S Cell Cycle Progression in Androgen-Independent Prostate Cancer |
|
