|
| Status |
Public on Feb 01, 2011 |
| Title |
Pt1 isolated subcutaneous adipocytes, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Isolated subcutaneous adipocytes, patient 1
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: isolated subcutaneous adipocytes
patient: 1
|
| Treatment protocol |
Adipocytes isolated by digestion with collagenase for 1 hour, 37C, 220 rpm shaker. Floating fraction collected.
|
| Growth protocol |
No growth was involved; these are operating room specimens.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard protocols from 1.2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA was hybridized to the GeneChip Human Genome U133 Plus 2.0 Array following standard protocols.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Pt1_sqAds_2
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Dec 28, 2010 |
| Last update date |
Feb 01, 2011 |
| Contact name |
Frank H Lau |
| Organization name |
Center for Regenerative Medicine
|
| Street address |
185 Cambridge St - CPZN 4500
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02130 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE26339 |
Expression data from pericardial and subcutaneous adipose tissue and adipocytes |
|
