|
| Status |
Public on Mar 05, 2012 |
| Title |
mES, p53-/-, Ctr, rep3 |
| Sample type |
RNA |
| |
|
| Source name |
mES, p53-/-, Ctr
|
| Organism |
Mus musculus |
| Characteristics |
background/strain: 129X1 x 129S1
cell type: embryonic stem cells (mES)
genotype: p53-/-
agent: untreated
|
| Treatment protocol |
Cells were either untreated or treated with 0.5 micromolar adriamycin for 8 hours in growth medium.
|
| Growth protocol |
Cells were grown in mESC growth medium, Knockout DMEM (KO-DMEM), 15% Fetal Bovine Serum, 100mM MEM non-essential amino acids, 0.1mM 2-mercaptoethanol, 2mM L-glutamine and 1X penicillin-streptomycin (All from Invitrogen) plus 1000 units/ml leukemia inhibitory factor (Lif, Millipore).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNAs were extracted using the RNeasy mini kit (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Affymetrix Whole Transcript Sense Target Labeling kit per manufacturer’s protocol.
|
| |
|
| Hybridization protocol |
Samples were hybridized to the Affymetrix Mouse Gene ST 1.0 GeneChip.
|
| Scan protocol |
Arrays were scanned on the Affymetrix GeneChip scanner 3000.
|
| Description |
JH27
|
| Data processing |
Data were processed with Partek Genomics Suite v6.4. Signal intensities were RMA normalized.
Probe group file: MoGene-1_0-st-v1.r4.pgf
Meta-probeset file: MoGene-1_0-st-v1.r4.mps
|
| |
|
| Submission date |
Dec 29, 2010 |
| Last update date |
Aug 20, 2013 |
| Contact name |
Jing Huang |
| E-mail(s) |
huangj3@mail.nih.gov
|
| Organization name |
National Cancer Institute
|
| Lab |
Cancer Biology and Genetics
|
| Street address |
37 Convent Dr. 37/3140
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
MD 20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (2) |
| GSE26360 |
Genome-wide analysis revealed a crosstalk between p53 and the pluripotent gene networks in mouse embryonic stem cells (expression) |
| GSE26362 |
Whole-genome study reveals distinct mechanisms used by p53 to regulate activated and repressed genes in embryonic stem cells |
|
