|
| Status |
Public on Nov 18, 2022 |
| Title |
m6a immunoprecipitation |
| Sample type |
SRA |
| |
|
| Source name |
whole uterus
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
tissue: whole uterus (GD4 uterus and GD8 uterus were pooled in equal amounts)
antibody: anti-m6a (Synaptic Systems, #202003)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TRIzol reagent (Invitrogen) was used to extract total RNAs according to manufacturers’ instructions.
Purified RNA was used for RNA-seq library generation with NEBNext® Ultra™ II Directional RNA Library Prep Kit (NEB).
|
| |
|
| Library strategy |
OTHER |
| Library source |
transcriptomic |
| Library selection |
other |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
library strategy: MeRIP-seq
After quality control, clean RNA-seq data were aligned to the reference genome (UCSC mm10) with Hisat2 software v2.2.1
Peaks were called uing MACS v3.0.0a7 with the significance cut-off q-value <=0.05
Assembly: mm10
Supplementary files format and content: narrowPeak (except for Input sample)
|
| |
|
| Submission date |
Aug 18, 2022 |
| Last update date |
Nov 18, 2022 |
| Contact name |
Ji-Long Liu |
| E-mail(s) |
jilongliu@scau.edu.cn
|
| Organization name |
South China Agricultural University
|
| Street address |
483 Wushan Rd
|
| City |
Guangzhou |
| ZIP/Postal code |
510642 |
| Country |
China |
| |
|
| Platform ID |
GPL24247 |
| Series (1) |
| GSE211521 |
Global m6A-modified mRNAs in the uterus of wild-type mouse |
|
| Relations |
| BioSample |
SAMN30383302 |
| SRA |
SRX17131969 |
