|
| Status |
Public on Jan 04, 2011 |
| Title |
Male8_TRE_log2 |
| Sample type |
RNA |
| |
|
| Source name |
FACS sorted P4 ORS cells
|
| Organism |
Mus musculus |
| Characteristics |
tissue: skin
strain: FVB/N
genotype: TRE-miR-125b/K14-H2BGFP
age: P4
Sex: male
treatment: Dox 24hrs
|
| Treatment protocol |
Hair follicle cells were isolated from P4 Backskin (Dox since P3 for 24hrs) of DTG (K14-rtTA/TRE-miR-125b/K14-H2BGFP), TRE (TRE-miR-125b/K14-H2BGFP), KrtA (K14-rtTA/K14-H2BGFP) as following: interfollicular epidermis sheet was pealed from hair follicle & dermis after dispase treatment.The hair follicle & dermis were first digested by collagenase (Sigma). Intact hair follicles were separated from dermal cells by low speed spinning (20g). The hair follicles were then digested by Trypsin and filtered by 40 µm cell strainers. The isolated hair follicle cells were FACS sorted. During FACS, cells were first gated against CD34 (endothelial cells), CD45 (immune cells), CD114 (melanocytes) and DAPI (dead cells). ORS cells were sorted from the remaining cells as α6HiGFPHi
|
| Growth protocol |
Standard mouse growth protocol
|
| Extracted molecule |
total RNA |
| Extraction protocol |
miRNeasy kit (Qiagen) extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Labeled cRNA samples were hybridized to MOE 430 2.0 mouse expression arrays which were processed and analyzed according to standard Affymetrix protocols.
|
| Scan protocol |
Standard Affymetrix protocol
|
| Description |
Gene expression data from FACS sorted P4 ORS
|
| Data processing |
The data were analyzed with RMA using Affymetrix expression console default analysis settings. All displayed signals were log2 transformed
|
| |
|
| Submission date |
Jan 03, 2011 |
| Last update date |
Jan 04, 2011 |
| Contact name |
liang zhang |
| E-mail(s) |
liangz@colorado.edu
|
| Organization name |
university of colorado at boulder
|
| Street address |
University of Colorado at Boulder, Han lab, Department of MCD Biology, Campus box 0347
|
| City |
Boulder |
| State/province |
CO |
| ZIP/Postal code |
80309-0347 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (2) |
| GSE26394 |
Gene Expression data of P4 stage hair follicle ORS cells from DTG (K14-rtTA,TRE-miR-125b) and control littermates |
| GSE26396 |
Specific MicroRNAs Are Preferentially Expressed by Skin Stem Cells To Balance Self-Renewal and Early Lineage Commitment |
|
