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Status
Public on Jan 05, 2011
Title
H3K27ac, rep 1
Sample type
genomic
Source name
S.pombe wt
Organism
Schizosaccharomyces pombe
Characteristics
antibody: H3K27ac strain: Hu303(972h-)
Growth protocol
YES media at 30 degree
Extracted molecule
genomic DNA
Extraction protocol
Robyr, D. and Grunstein, M. (2003) Genomewide histone acetylation microarrays. Methods, 31, 83-89
Label
Cy5
Label protocol
Spotted microarrays were hybridized using Cy3 and Cy5
Hybridization protocol
Robyr, D. and Grunstein, M. (2003) Genomewide histone acetylation microarrays. Methods, 31, 83-89
Scan protocol
ImaGene 4.2
Description
ChIP on chip
Data processing
Genespring 7.3
Submission date
Jan 04, 2011
Last update date
Jan 05, 2011
Contact name
Karl Ekwall
E-mail(s)
karl.ekwall@ki.se
Phone
+46 8 6089133
Organization name
Karolinska Inst
Street address
Alfred Nobels Alle 7
City
Stockholm
ZIP/Postal code
S-141 89
Country
Sweden
Platform ID
GPL11381
Series (1)
GSE11511
Identification of histone codes and crosstalk in fission yeast
Data table header descriptions
ID_REF
VALUE
Spotted microarrays were hybridized using Cy3 and Cy5. Each channel was then processed separately. Intensity values were normalized by scaling. Values were divided by the median value for each channel and sample and multiplied by 500.
Data table
ID_REF
VALUE
1
3506.8
2
4063.2
3
2292.7
4
2334.2
5
3649.5
6
2697.8
7
851.4
8
765.6
9
335.6
10
287.3
11
10192.4
12
10872.2
13
99.4
14
103.4
15
95.8
16
92.1
17
93.9
18
93.0
19
92.6
20
89.4
Total number of rows: 22176 Table truncated, full table size 251 Kbytes .Supplementary file
Size
Download
File type/resource
GSM648730_US23502366_J120E_33_S01_Red.txt.gz
1.9 Mb
(ftp) (http)
TXT
Processed data included within Sample table
Processed data are available on Series record