|
| Status |
Public on Sep 27, 2023 |
| Title |
CK 1 |
| Sample type |
SRA |
| |
|
| Source name |
leaves, shoots and roots
|
| Organism |
Lumnitzera littorea |
| Characteristics |
tissue: leaves, shoots and roots
age: 1 year
genotype: wt
treatment: control
|
| Treatment protocol |
The seedlings were divided into two groups: one group were grown under the temperature of 25°C day/23°C night (normal condition) for 48 h, while the other were grown under the temperature of 8°C day/5°C night (chilling stress).
|
| Growth protocol |
One-year-old seedlings of L. littorea with 5–6 true leaves and a height of 30 ± 2 cm were chosen as experimental materials, and grown at controlled climatic conditions: day/night photoperiod, 12/12 h; 50% relative humidity and 80% relative light intensity during the day; and 70% relative humidity and zero relative light intensity during the night.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs was extracted from the leaves, shoots and roots of each plant using EASYspin Plus Complex Plant RNA Kit.
Illumina libraries were prepared following manufacturer’s instruction
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
Qualities of de-barcoded raw sequence reads were checked by fastqc.
Reads of each sample were aligned to the reference genome of Lumnitzera littorea via hisat2 program
Gene counts were obtained using featureCounts.
Supplementary files format and content: One csv file containing raw count values for each sample
|
| |
|
| Submission date |
Aug 20, 2022 |
| Last update date |
Sep 27, 2023 |
| Contact name |
Yuchen Yang |
| E-mail(s) |
yangych68@mail.sysu.edu.cn
|
| Organization name |
Sun Yat-sen University
|
| Street address |
Xingangxi Road 135
|
| City |
Guangzhou |
| State/province |
Guangdong |
| ZIP/Postal code |
510275 |
| Country |
China |
| |
|
| Platform ID |
GPL32585 |
| Series (1) |
| GSE211695 |
Unravelling Chilling-stress Resistance Mechanisms in Endangered Mangrove Plant Lumnitzera littorea (Jack) Voigt |
|
| Relations |
| SRA |
SRX13497998 |
| BioSample |
SAMN24369535 |
