|
| Status |
Public on Aug 25, 2022 |
| Title |
Vector Control polysome 2 |
| Sample type |
RNA |
| |
|
| Source name |
THP1
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: THP1 (Acute monocytic leukemia)
cell state: proliferating (with serum)
gender: Male
|
| Treatment protocol |
No treatment
|
| Growth protocol |
RPMI1640 media supplemented with 10% fetal bovine serum (FBS), 2 mM L-Glutamine, 100 μg/mL streptomycin and 100 U/ml penicillin at 37°C in 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA and polysome associated RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotin labeling was performed using the WT Plus reagent kit (Affymetrix, Santa Clara, CA) according to the manufacturer's protocol.
|
| |
|
| Hybridization protocol |
The labeled, fragmented DNA was hybridized to the Affymetrix Human Gene 2.0 ST Array for 18 hours in a GeneChip Hybridization oven 640 at 45oC with rotation (60 rpm). The hybridized samples were washed and stained using an Affymetrix fluidics station 450.
|
| Scan protocol |
After staining, microarrays were immediately scanned using an Affymetrix GeneArray Scanner 3000 7G Plus
|
| Description |
Polysome associated RNA from vector control cells (replicate 2)
|
| Data processing |
HuGene 2.0 ST CEL files were normalized to produce gene-level expression values with the Robust Multiarray Average (RMA) using the affy R package (version 1.62.0) and Entrez Gene-specific R packages (version 24.0.0) from the Molecular and Behavioral Neuroscience Institute (Brainarray) at the University of Michigan
|
| |
|
| Submission date |
Aug 24, 2022 |
| Last update date |
Aug 25, 2022 |
| Contact name |
Shobha Vasudevan |
| E-mail(s) |
Vasudevan.Shobha@mgh.harvard.edu
|
| Organization name |
Massachusetts General Hospital
|
| Department |
Cancer Center
|
| Street address |
185 Cambridge St
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02114 |
| Country |
USA |
| |
|
| Platform ID |
GPL27949 |
| Series (1) |
| GSE211932 |
Transcriptome and translatome analysis of constitutively overexpression of FXR1 and control THP1 cells |
|
