|
| Status |
Public on Aug 27, 2022 |
| Title |
bta-miR-200b, mimic, Group3 |
| Sample type |
SRA |
| |
|
| Source name |
BEND
|
| Organism |
Bos taurus |
| Characteristics |
cell line: BEND
cell type: Bovine endometrial epithelial cells
transfection: bta-miR-200b mimic
genotype: bta-miR-200b overexpression
treatment: Cell apoptosis
|
| Treatment protocol |
After 70%-80% cell fusion, the cells were transfected with bta-miR-200b mimic and mimic NC
|
| Growth protocol |
BEND were maintained in DMEM supplemented with 10% fetal bovine serum (FBS) and antibiotics in humidified atmosphere with 5% CO2 at 37C
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested using TRIzol reagent (Invitrogen)
The libraries were constructed using TruSeq Stranded mRNA LT Sample Prep Kit (Illumina, USA) according to the manufacturer’s instructions
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
HiSeq X Ten |
| |
|
| Description |
sample M3
|
| Data processing |
The adaptor sequences and low-quality reads were removed using Trimmomatic
Clean reads were mapped to the bovine genome (ARS-UCD 1.2) using HISAT2
Htseq package was used to get gene counts
Assembly: ARS-UCD 1.2
Supplementary files format and content: Excel file includes RPKM values for each Sample
|
| |
|
| Submission date |
Aug 24, 2022 |
| Last update date |
Aug 27, 2022 |
| Contact name |
Shijie Lyu |
| Organization name |
Henan Academy of Agricultural Sciences
|
| Street address |
Huayuan Road 116
|
| City |
Zhengzhou |
| ZIP/Postal code |
450002 |
| Country |
China |
| |
|
| Platform ID |
GPL24230 |
| Series (1) |
| GSE211959 |
RNA-seq analysis of bta-miR-200b overexpression in bovine endometrial epithelial cells |
|
| Relations |
| BioSample |
SAMN30478323 |
| SRA |
SRX17238570 |
