|
| Status |
Public on Jun 30, 2023 |
| Title |
ICC077pre |
| Sample type |
RNA |
| |
|
| Source name |
serum
|
| Organism |
Homo sapiens |
| Characteristics |
disease/diagnosis: Cholangiocellular carcinoma
sample collection time: before operation
molecule subtype: miRNA
|
| Treatment protocol |
Blood was collected before surgical treatment.
|
| Growth protocol |
Serum was collected from the blood.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from serum was extracted commercially available kit (miReasy mini kit (Qiagen)).
|
| Label |
Cy5
|
| Label protocol |
Extracted total RNA was labeled with Cy5 using the 3D-Gene miRNA labeling kit (Toray Industries Inc., Tokyo, Japan).
|
| |
|
| Hybridization protocol |
Hybridized for 16 h at 32 C with rotary shake (250 rpm). Hybridization buffer and washing protocol was followed by the protocol supplied by TORAY Industries, Inc..
|
| Scan protocol |
3D-Gene Scanner ((Toray Industries Inc., Tokyo, Japan) was used for scanning. Images were quantified using Extraction(Toray Industries Inc., Tokyo, Japan).
|
| Description |
SH8X230_Cell2
|
| Data processing |
The raw data of each spot was normalized by substitution with a mean intensity of the background signal determined by all blank spots’ signal intensities of 95%
|
| |
|
| Submission date |
Aug 29, 2022 |
| Last update date |
Jun 30, 2023 |
| Contact name |
Satoko Takizawa |
| E-mail(s) |
satoko.takizawa.f3@mail.toray
|
| Phone |
+81-467-32-9351
|
| Organization name |
Toray Industries, Inc.
|
| Street address |
6-10-1, Tebiro
|
| City |
Kanagawa |
| State/province |
Kamakura |
| ZIP/Postal code |
2480036 |
| Country |
Japan |
| |
|
| Platform ID |
GPL21263 |
| Series (1) |
| GSE212211 |
Development of blood biomarkers that distinguish pancreatic cancer from biliary tract cancer |
|
