|
Status |
Public on Jan 01, 2012 |
Title |
HCG1/BG14 - repeat 3 - mAdbID:106672 |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
WT BG14 Control - Cy3
|
Organism |
Nakaseomyces glabratus |
Characteristics |
strain: BG14 developmental stage: log-phase culture genotype/variation: Wild Type
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol Extraction Protocol Other: Total RNA was isolated from log-phase culture of C. glabrata grown in YPD using Trizole (Invitrogen) and RNeasy MiniElute cleanup kit (Qiagen).
|
Label |
cy3
|
Label protocol |
Cy3 Labeling Protocol Other: Ten micrograms of total RNA were used for each labeling. The RNA was reversed transcribed to cDNA to incorporate the fluorescent Cy3-dUTP (GE Health Care).
|
|
|
Channel 2 |
Source name |
mt HCG1 - Cy5
|
Organism |
Nakaseomyces glabratus |
Characteristics |
strain: HCG1 developmental stage: log-phase culture genotype/variation: ure2 mutant
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol Extraction Protocol Other: Total RNA was isolated from log-phase culture of C. glabrata grown in YPD using Trizole (Invitrogen) and RNeasy MiniElute cleanup kit (Qiagen).
|
Label |
cy5
|
Label protocol |
Cy5 Labeling Protocol Other: Ten micrograms of total RNA were used for each labeling. The RNA was reversed transcribed to cDNA to incorporate the fluorescent Cy5-dUTP (GE Health Care).
|
|
|
|
Hybridization protocol |
Agilent Hybridization Other: According to the manufacture's recommended protocol (Two-Color Microarray-Based Gene Expression Analysis, Agilent).
|
Scan protocol |
Agilent Scanning Protocol Other: Arrays were scanned at 5um resolution and 100/10% PMT XDR on an Agilent DNA Microarray Scanner G2505B.
|
Description |
mAdb experiment ID: 106672
|
Data processing |
Agilent Data Processing Protocol Calculation Method: Images were auto gridded, analyzed and data extracted using Agilent Feature Extraction Software. Spot values were normalized using the default linear-lowess normalization.
|
|
|
Submission date |
Jan 13, 2011 |
Last update date |
Jan 01, 2012 |
Contact name |
Huei-Fung Tsai |
E-mail(s) |
HT28T@NIH.GOV
|
Phone |
301-496-8993
|
Fax |
301-480-0512
|
Organization name |
NIH/NIAID
|
Department |
LCID
|
Lab |
CMS
|
Street address |
10 Center Dr. Bldg. 10 Rm. 11N228
|
City |
Bethesda |
State/province |
MD |
ZIP/Postal code |
20892 |
Country |
USA |
|
|
Platform ID |
GPL10325 |
Series (2) |
GSE26612 |
[URE3] Prion formation by the Candida albicans Ure2p (but not by C. glabrata Ure2p)-CgUre2 |
GSE26620 |
[URE3] Prion formation by the Candida albicans Ure2p (but not by C. glabrata Ure2p) |
|