|
| Status |
Public on Jan 01, 2013 |
| Title |
NIH 3T3_SapKD_biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
NIH 3T3 cells stably expressing Sap-1 shRNA stimulated with serum
|
| Organism |
Mus musculus |
| Characteristics |
cell line: NIH 3T3 cells
genotype/variation: Sap-1 knockdown
|
| Treatment protocol |
Cells were serum starved overnight (serum-free DMEM), then stimulated with 10% bovine serum for one hour, then lysed.
|
| Growth protocol |
Cells were cultured in high-glucose DMEM containing 10% bovine serum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Qiagen RNeasy mini kits according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 0.1 ug total RNA using the Ambion WT Expression Kit (GeneChip Expression Analysis Technical Manual, www.affymetrix.com).
|
| |
|
| Hybridization protocol |
Following fragmentation, 5 ug of cRNA were hybridized for 16 hr at 45 C on Mouse Gene 1.0ST Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 .
|
| Scan protocol |
GeneChips were scanned using the GeneArray Scanner 3000 7G.
|
| Description |
Gene expression data from serum-stimulated NIH 3T3 cells expressing Sap-1 shRNA.
|
| Data processing |
The data were analyzed with the RMA algorithm in Expression Console.
|
| |
|
| Submission date |
Jan 14, 2011 |
| Last update date |
Jan 01, 2013 |
| Contact name |
Michele Wozniak |
| E-mail(s) |
wozniak@seas.upenn.edu
|
| Phone |
215-746-1750
|
| Organization name |
University of Pennsylvania
|
| Department |
Bioengineering
|
| Lab |
Christopher Chen
|
| Street address |
210 S. 33rd St., 510 Skirkanich
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE26640 |
Expression data from serum-stimulated NIH 3T3 cells stably expressing control (pLKO.1) vector or shRNAs targeting Net or Sap-1 |
|
