|
Status |
Public on Jan 20, 2011 |
Title |
Biomaterial_BL_002_SI |
Sample type |
RNA |
|
|
Source name |
Endemic Burkitt lymphoma
|
Organism |
Homo sapiens |
Characteristics |
tissue: Lymph-node biopsy genotype: African patient age: Children
|
Treatment protocol |
Samples were taken at diagnosis from untreated patients
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2-5 micrograms total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
|
|
Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
Scan protocol |
GeneChips were scanned using the Affymetrix Scanner G3000.
|
Description |
Gene expression data from patient's sample
|
Data processing |
The data were analyzed with Affymetrix GCOS. Normalization: RMA. Baseline Transformation: Median of all samples.
|
|
|
Submission date |
Jan 18, 2011 |
Last update date |
Jan 20, 2011 |
Contact name |
pier paolo piccaluga |
E-mail(s) |
pierpaolo.piccaluga@unibo.it
|
Phone |
+390516364043
|
Fax |
+390516364037
|
Organization name |
University of Bologna
|
Department |
Hematology and Oncological Sciences "L. and A. Serà gnoli"
|
Lab |
Molecular Pathology
|
Street address |
via massraenti 9
|
City |
Bologna |
ZIP/Postal code |
40138 |
Country |
Italy |
|
|
Platform ID |
GPL570 |
Series (1) |
GSE26673 |
Expression data from Burkitt lymphoma cases |
|