|
| Status |
Public on Oct 25, 2023 |
| Title |
Skin_biopsy_EL_case3 |
| Sample type |
RNA |
| |
|
| Source name |
archival skin biopsy
|
| Organism |
Canis lupus familiaris |
| Characteristics |
disease status: EL
disease details: primary tumor
|
| Treatment protocol |
N/A
|
| Growth protocol |
N/A
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from 30 micron curls from archival skin biopsies using Qiagen FFPE RNEasy kit with Deparaffinization solution per the manufacturer's instructions.
|
| Label |
fluorescent probe
|
| Label protocol |
Probe labeling was performed per Nanostring manufacturer's protocol
|
| |
|
| Hybridization protocol |
Hybridizations were performed per Nanostring manufacturer's protocol overnight for 18h in a BioRad C1000 touch thermocycler
|
| Scan protocol |
Cartridges were run on a Nanostring Sprint machine
|
| Description |
10yo_spayedFemale_ShihTzu
|
| Data processing |
Data were analyzed with nSolver software version 4.0 and ROSALIND online software.
|
| |
|
| Submission date |
Sep 10, 2022 |
| Last update date |
Oct 25, 2023 |
| Contact name |
Jillian M Richmond |
| E-mail(s) |
jillian.richmond@umassmed.edu
|
| Organization name |
UMass Chan Medical School
|
| Department |
Dermatology
|
| Street address |
364 Plantation St
|
| City |
Worcester |
| State/province |
Massachusetts |
| ZIP/Postal code |
01605 |
| Country |
USA |
| |
|
| Platform ID |
GPL32655 |
| Series (2) |
| GSE213086 |
Gene expression analysis in canine epitheliotropic lymphoma - validation cohort |
| GSE213087 |
Gene expression analysis in canine epitheliotropic lymphoma |
|
