|
Status |
Public on Oct 25, 2023 |
Title |
Skin_biopsy_ID_case5 |
Sample type |
RNA |
|
|
Source name |
archival skin biopsy
|
Organism |
Canis lupus familiaris |
Characteristics |
disease status: ID disease details: Hepatocutaneous syndrome
|
Treatment protocol |
N/A
|
Growth protocol |
N/A
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was isolated from 30 micron curls from archival skin biopsies using Qiagen FFPE RNEasy kit with Deparaffinization solution per the manufacturer's instructions.
|
Label |
fluorescent probe
|
Label protocol |
Probe labeling was performed per Nanostring manufacturer's protocol
|
|
|
Hybridization protocol |
Hybridizations were performed per Nanostring manufacturer's protocol overnight for 18h in a BioRad C1000 touch thermocycler
|
Scan protocol |
Cartridges were run on a Nanostring Sprint machine
|
Description |
11yo_neuteredMale_ManchesterTerrier
|
Data processing |
Data were analyzed with nSolver software version 4.0 and ROSALIND online software.
|
|
|
Submission date |
Sep 10, 2022 |
Last update date |
Oct 25, 2023 |
Contact name |
Jillian M Richmond |
E-mail(s) |
jillian.richmond@umassmed.edu
|
Organization name |
UMass Chan Medical School
|
Department |
Dermatology
|
Street address |
364 Plantation St
|
City |
Worcester |
State/province |
Massachusetts |
ZIP/Postal code |
01605 |
Country |
USA |
|
|
Platform ID |
GPL32655 |
Series (2) |
GSE213086 |
Gene expression analysis in canine epitheliotropic lymphoma - validation cohort |
GSE213087 |
Gene expression analysis in canine epitheliotropic lymphoma |
|