|
Status |
Public on Sep 21, 2005 |
Title |
HSC_collagen_3 |
Sample type |
RNA |
|
|
Source name |
cord blood
|
Organism |
Homo sapiens |
Characteristics |
CD34+ cells isolated from umbilical cord blood, cultured in collagen I gels
|
Extracted molecule |
total RNA |
Extraction protocol |
RNeasy Mini Kit
|
Label |
Biotin/Streptavidin Phycoerythrin
|
Label protocol |
Affymetrix GeneChip Eukaryotic Small Sample Target Labeling Assay Version II, starting the first amplification cycle with 100ng total RNA
|
|
|
Hybridization protocol |
Standard Affymetrix hybridization protocol for eukaryotic samples
|
Scan protocol |
Affymetrix standard procedure
|
Description |
further details in Oswald et al. "Gene expression profiling of CD34+ hematopoietic cells expanded in a collagen I matrix"
|
Data processing |
Affymetrix MAS 5.0, global scaling strategy, TGT 500
|
|
|
Submission date |
Jul 26, 2005 |
Last update date |
Sep 21, 2005 |
Contact name |
Martin Bornhaeuser |
E-mail(s) |
martin.bornhaeuser@uniklinikum-dresden.de
|
Phone |
++49-351-458-4704
|
Fax |
++49-351-458-5389
|
Organization name |
Universitätsklinikum Carl Gustav Carus
|
Department |
Med. Klinik und Poliklinik
|
Street address |
Fetscherstrasse 74
|
City |
Dresden |
ZIP/Postal code |
01307 |
Country |
Germany |
|
|
Platform ID |
GPL96 |
Series (1) |
GSE3003 |
Gene expression profiling of CD34+ hematopoietic cells expanded in a collagen I matrix |
|