|
| Status |
Public on Sep 14, 2022 |
| Title |
SingleCellM1 |
| Sample type |
SRA |
| |
|
| Source name |
tumour
|
| Organism |
Mus musculus |
| Characteristics |
age: 8-9 weeks
gender: f
extract protocol: gentleMACS
tumour type: AB1-HA
strain: balb/c
timepoint: d0
|
| Treatment protocol |
N/A
|
| Growth protocol |
Subcutaneous tumour
|
| Extracted molecule |
total RNA |
| Extraction protocol |
4 samples with gentleMACS system (Miltenyi Biotec) and other 4 with via frozen tumour dissociation with Trizol followed by chloroform extraction and purification using RNeasy MinElute
Illumina 50bp single-end sequencing
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
read alignment with Kallisto
count quantification with sleuth
sleuth_to_matrix
Assembly: GRCm38.p6
Supplementary files format and content: txt files in compressed gz format
|
| |
|
| Submission date |
Sep 13, 2022 |
| Last update date |
Sep 14, 2022 |
| Contact name |
Wee Loong Chin |
| Organization name |
National Centre For Asbestos Related Diseases
|
| Street address |
Level 5, Qq Block, Qeii Medical Centre 6 Verdun St, Nedlands
|
| City |
Perth |
| ZIP/Postal code |
6009 |
| Country |
Australia |
| |
|
| Platform ID |
GPL13112 |
| Series (2) |
| GSE153943 |
Dynamic IFNβ signalling underlies treatment response to immune checkpoint therapy in cancer |
| GSE213296 |
Time-course bulk RNAseq and single-cell RNAseq of murine AB1 mesothelioma and Renca renal cancer following immune checkpoint therapy |
|
| Relations |
| BioSample |
SAMN30838596 |
| SRA |
SRX17556763 |
