|
| Status |
Public on Mar 24, 2011 |
| Title |
NBEC BRCA2 RNAi |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
NBEC pSuper Retro Vector
|
| Organism |
Homo sapiens |
| Characteristics |
time: day 5
transfection: NBEC pSuper Retro Vector
cell type: primary normal breast epithelial cells
|
| Treatment protocol |
NBEC were infected with virus produced from retro vector pMSCV-miR1245 or empty pMSCV vector; or pSuper Retro BRCA2 shRNA, or pSuper Retro empty vector.
|
| Growth protocol |
Primary normal breast epithelial cells (NBEC) were collected from the mammoplasty material of a 30-year-old woman at the Department of Plastic Surgery, the First Affiliated Hospital of Sun Yat-sen University (P. R. China), in accordance with rules and regulations concerning ethical issues on research use of human subjects in China, and were cultured in the Keratinocyte serum-free medium (Invitrogen, Carlsbad, CA) supplemented with epithelial growth factor, bovine pituitary extract and antibiotics (120 µg/mL streptomycin and 120 µg/mL penicillin).
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
After 2 days infection and 3 days culture, genomic DNA was isolated using the QIAamp DNA Mini Kit (QIAGENE, Valencia, CA).
|
| Label |
Cy3
|
| Label protocol |
Label, hybridization and scanning was performed in shanghai biochip corporation (http://www.shbiochip.com/) following standard Agilent protocol.
|
| |
|
| Channel 2 |
| Source name |
NBEC pSuper Retro BRCA2 RNAi
|
| Organism |
Homo sapiens |
| Characteristics |
time: day 5
infection: NBEC pSuper Retro BRCA2 RNAi retro vector
cell type: primary normal breast epithelial cells
|
| Treatment protocol |
NBEC were infected with virus produced from retro vector pMSCV-miR1245 or empty pMSCV vector; or pSuper Retro BRCA2 shRNA, or pSuper Retro empty vector.
|
| Growth protocol |
Primary normal breast epithelial cells (NBEC) were collected from the mammoplasty material of a 30-year-old woman at the Department of Plastic Surgery, the First Affiliated Hospital of Sun Yat-sen University (P. R. China), in accordance with rules and regulations concerning ethical issues on research use of human subjects in China, and were cultured in the Keratinocyte serum-free medium (Invitrogen, Carlsbad, CA) supplemented with epithelial growth factor, bovine pituitary extract and antibiotics (120 µg/mL streptomycin and 120 µg/mL penicillin).
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
After 2 days infection and 3 days culture, genomic DNA was isolated using the QIAamp DNA Mini Kit (QIAGENE, Valencia, CA).
|
| Label |
Cy5
|
| Label protocol |
Label, hybridization and scanning was performed in shanghai biochip corporation (http://www.shbiochip.com/) following standard Agilent protocol.
|
| |
|
| |
| Hybridization protocol |
Label, hybridization and scanning was performed in shanghai biochip corporation (http://www.shbiochip.com/) following standard Agilent protocol.
|
| Scan protocol |
Label, hybridization and scanning was performed in shanghai biochip corporation (http://www.shbiochip.com/) following standard Agilent protocol.
|
| Description |
Positive Control
|
| Data processing |
Data normalization and transformation was performed on Agilent Genomic Workbench 6.5 (Agilent) in shanghai biochip corporation.
|
| |
|
| Submission date |
Jan 19, 2011 |
| Last update date |
Mar 24, 2011 |
| Contact name |
Mengfeng Li |
| E-mail(s) |
limf@mail.sysu.edu.cn
|
| Organization name |
Sun Yat-sen University
|
| Department |
Zhongshan school of medicine
|
| Lab |
Joint Lab of Prof. Mengfeng Li and Prof. Jun Li
|
| Street address |
74# zhongshan 2 Rd.
|
| City |
Guangzhou |
| State/province |
Guangdong |
| ZIP/Postal code |
510080 |
| Country |
China |
| |
|
| Platform ID |
GPL10123 |
| Series (1) |
| GSE26715 |
miR-1245 induce genomic instability |
|
