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Sample GSM657958 Query DataSets for GSM657958
Status Public on Jan 20, 2011
Title WT_2
Sample type RNA
 
Source name Wild Type Mouse 2
Organism Mus musculus
Characteristics gender: male
cell type: dendritic cells
genotype/variation: Wildtype
Treatment protocol Cells were purified from digested prostates by magnetic beads >90% purity was confirmed
Extracted molecule total RNA
Extraction protocol RNA was isolated using the qiagen RNAeasy mini spin colomn according to manufacturer's instuctions.
RNA quality was checked on Agilent Bioanalyzer. All samples used for microarray analysis have high quality score (RIN >9).
Label biotin
Label protocol 200 ng of RNA was reverse transcribed with random hexamer primer, amplified and terminal labeled with biotin using Affymetrix Whole Transcript Sense Target Labeling kit according to manufacturer’s protocol
 
Hybridization protocol replicates of each group were hybridized to Affymetrix mouse Gene ST 1.0 GeneChip in Affymetrix hybridization oven at 45C, 60rpm for 16 hrs
Scan protocol Wash and stain were performed on Affymetrix Fluidics Station 450 and scanned on Affymetrix GeneChip scanner 3000
Description Dendritic cells Wildtype Mouse 2
Data processing Data were collected using Affymetrix AGCC software. Statistical and clustering analysis was performed with Partek Genomics Suite software using RMA normalization algorithm.
 
Submission date Jan 19, 2011
Last update date Jan 20, 2011
Contact name Stephanie Kaye Watkins
E-mail(s) watkinssk@mail.nih.gov
Phone 301-846-6871
Fax 301-846-6005
Organization name NCI-Frederick
Department CIP/LMI
Lab Tumor Immunity and Tolerance
Street address 1050 Boyles St.
City Frederick
State/province MD
ZIP/Postal code 21702
Country USA
 
Platform ID GPL10740
Series (1)
GSE26719 Gene expression analysis of dendritic cells from normal or tumor bearing prostates

Data table header descriptions
ID_REF
VALUE Log2 RMA values

Data table
ID_REF VALUE
10338068 7.65024
10338069 3.53188
10338070 3.97156
10338071 3.25801
10338072 3.89398
10338073 6.98781
10338074 6.65776
10338075 4.54831
10338076 5.759
10338077 7.9042
10338078 4.78747
10338079 4.1047
10338080 5.45127
10338081 6.3142
10338082 4.97407
10338083 2.84578
10338084 5.44581
10338085 6.35303
10338086 6.51185
10338087 4.21159

Total number of rows: 241419

Table truncated, full table size 3983 Kbytes.




Supplementary file Size Download File type/resource
GSM657958.CEL.gz 3.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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