|
| Status |
Public on May 20, 2011 |
| Title |
DKO replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
DKO cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: cancer cell line HCT116
genotype/variation: DMNT-1 and 3B double knock out (DKO)
|
| Treatment protocol |
No treatments were given to the cells.
|
| Growth protocol |
Both HCT116 and DKO cells were cultured in McCoy's 5A medium supplemented with 10% fetal bovine serum at 37oC, 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with Trizol reagent, followed by DNase I treatment. RNA quality and concentration was measure using the nanodrop spectrophtometer.
|
| Label |
Cy3
|
| Label protocol |
Standard Illumina hybridization protocol
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
DKO-2
replicate 2
|
| Data processing |
The data was quantile normalized and analyzed using the Illumina GenomeStudio v2009.1.
|
| |
|
| Submission date |
Jan 24, 2011 |
| Last update date |
May 20, 2011 |
| Contact name |
Angela Ting |
| E-mail(s) |
tinga@ccf.org
|
| Organization name |
Cleveland Clinic
|
| Department |
Genomic Medicine
|
| Street address |
9500 Euclid Ave/NE50
|
| City |
Cleveland |
| State/province |
Ohio |
| ZIP/Postal code |
44195 |
| Country |
USA |
| |
|
| Platform ID |
GPL8179 |
| Series (1) |
| GSE26819 |
Identification and functional analysis of epigenetically silenced microRNAs in colorectal cancer cells |
|
