|
| Status |
Public on Jun 14, 2023 |
| Title |
WT, scRNAseq [JP1295] |
| Sample type |
SRA |
| |
|
| Source name |
whole kidney
|
| Organism |
Mus musculus |
| Characteristics |
cell type: WT
tissue: whole kidney
age: E18.5
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Single-cell suspensions were processed through Chromium 10X to generate single-cell cDNA libraries attached to microbeads
Microbeads were counted, and amplified by PCR, and the 3' end of the cDNA prepared for sequencing using a modified Nextera XT protocol
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic single cell |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
10x Genomics
|
| Data processing |
The demultiplexing, barcoded processing, gene counting and aggregation were made using the Cell Ranger software v6.1.2 (https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/latest/what-is-cell-ranger)
Assembly: mm10
Supplementary files format and content: Tab-separated values files and matrix files
|
| |
|
| Submission date |
Sep 23, 2022 |
| Last update date |
Jun 14, 2023 |
| Contact name |
Steve Potter |
| Organization name |
Cincinnati Children's Hospital Research Foundation
|
| Department |
Developmental Biology
|
| Street address |
240 Albert Sabin Way
|
| City |
Cincinnati |
| State/province |
Ohio |
| ZIP/Postal code |
45229 |
| Country |
USA |
| |
|
| Platform ID |
GPL24247 |
| Series (1) |
| GSE214024 |
Single-cell sequencing dissects the transcriptional identity of activated fibroblasts and identifies novel persistent distal tubular injury patterns in kidney |
|
| Relations |
| BioSample |
SAMN30983930 |
| SRA |
SRX17681859 |
