|
|
GEO help: Mouse over screen elements for information. |
|
Status |
Public on Oct 14, 2022 |
Title |
HL3, linseed oil, rep5 |
Sample type |
SRA |
|
|
Source name |
liver
|
Organism |
Anas platyrhynchos |
Characteristics |
breed: Runzhou white crest duck tissue: liver Sex: male treatment: linseed oil group treatment: ducks were fed by 2% linseed oil time: Day 28
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted by a Trizol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's protocol. RNA libraries for RNA-seq were prepared using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® following manufacturer's protocols.
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NovaSeq 6000 |
|
|
Data processing |
Illumina NovaSeq 6000 Clean reads were obtained by removing reads containing adapter, reads containing ploy-N and low quality reads from raw data. Clean data were mapped to the duck reference genome (https://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/015/476/345/GCF_015476345.1_ZJU1.0/GCF_015476345.1_ZJU1.0_genomic.fna.gz) by Hisat2 (version: 2.0.5). mRNA levels was quantified by the value of fragments per kilobase of exon per million mapped reads (FPKM). Assembly: ZJU1.0 Supplementary files format and content: tab-delimited text files include RPKM values for all Samples
|
|
|
Submission date |
Oct 02, 2022 |
Last update date |
Oct 14, 2022 |
Contact name |
Wang Laidi |
E-mail(s) |
gbchang1975@yzu.edu.cn
|
Phone |
18762321895
|
Organization name |
Yangzhou University
|
Street address |
East Wenhui Road
|
City |
Yangzhou |
State/province |
Jiangsu |
ZIP/Postal code |
225009 |
Country |
China |
|
|
Platform ID |
GPL28134 |
Series (1) |
GSE214632 |
Dietary Linseed Oil Affects the Polyunsaturated Fatty Acid and Transcriptome Profiles in the Livers and Breast Muscles of Ducks |
|
Relations |
BioSample |
SAMN31131703 |
SRA |
SRX17776889 |
Supplementary data files not provided |
SRA Run Selector |
Raw data are available in SRA |
Processed data are available on Series record |
|
|
|
|
|