|
Status |
Public on Dec 20, 2011 |
Title |
Affy_dic9-20_3_bm |
Sample type |
RNA |
|
|
Source name |
Bone marrow
|
Organism |
Homo sapiens |
Characteristics |
disease state: acute lymphoblastic leukemia tissue: bone marrow subtype: dic(9;20)
|
Extracted molecule |
total RNA |
Extraction protocol |
AllPrep DNA/RNA Mini Kit (Qiagen)
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared from two micrograms of total RNA
|
|
|
Hybridization protocol |
Biotinylated cRNAs were hybridized to the gene chips for 16 hours in a 45°C incubator, rotated at 60 rpm.
|
Scan protocol |
The GeneChips were then washed and stained using the Fluidics Station 450 and scanned using the GeneChip® Scanner 3000 7G
|
Data processing |
The data was normalized using the robust multi-array average (RMA) method (Irizarry et al. 2003)
|
|
|
Submission date |
Jan 25, 2011 |
Last update date |
May 17, 2013 |
Contact name |
Jessica Nordlund |
E-mail(s) |
jessica.nordlund@medsci.uu.se
|
Phone |
+46186114912
|
Organization name |
Uppsala University
|
Department |
Medical Sciences
|
Lab |
Molecular Medicine
|
Street address |
Academic Hospital, Entrance 70, 3rd floor
|
City |
Uppsala |
ZIP/Postal code |
75185 |
Country |
Sweden |
|
|
Platform ID |
GPL570 |
Series (2) |
GSE26865 |
Gene expression profiling of primary acute lymphoblastic leukemia (ALL) cells |
GSE26878 |
Digital gene and expression profiling of primary acute lymphoblastic leukemia (ALL) cells |
|
Relations |
Reanalyzed by |
GSM1143852 |