|
Status |
Public on Jan 26, 2011 |
Title |
skeletal muscle main population cells from fetal human subject 9 |
Sample type |
RNA |
|
|
Source name |
skeletal muscle main population cells from fetal human subject 9
|
Organism |
Homo sapiens |
Characteristics |
tissue of origin: skeletal muscle gestational age of subject in weeks: 14 weeks percentage (%) of sp cells: 2.05 percentage (%) of mp cells: 63.40 microarray hybridization date: 12/02/2002 microarray data quality: good
|
Treatment protocol |
None
|
Growth protocol |
Human samples were thawed and plated overnight in DMEM 20% FBS. SP and MP cells were isolated by FACS purification the next day.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted from sorted human fetal muscle side population (SP) and main population (MP) cells using the RNeasy kit (QIAGEN). Total RNA (100 ng) from 22 SP and 10 MP samples were subjected to two rounds of linear amplification using a MessageAmp kit (Ambion).
|
Label |
biotin
|
Label protocol |
Fragmented cRNA was biotin-labeled via standard Affymetrix labeling protocol.
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|
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Hybridization protocol |
Standard Affymetrix hybridization protocol for the Affymetrix human HG-U133A GeneChip for 20 µg of cRNA.
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Scan protocol |
Scanning is carried out on a Affymetrix GeneChip® Scanner 3000 7G scanner with autoloader. The Affymetrix GCOS v1.3 operating system controls the Model 3000 7G scanner and data acquisition functions. GCOS maintains the mediated first level data analysis and desktop data management for the entire GeneChip System.
|
Description |
skeletal muscle main population cells from fetal human subject 9
|
Data processing |
CEL files were inputted into Bioconductor/R to derive Affymetrix Microarray Suite version 5.0 (MAS 5.0) normalized output.
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|
|
Submission date |
Jan 26, 2011 |
Last update date |
Jan 26, 2011 |
Contact name |
Alvin T. Kho |
E-mail(s) |
alvin_kho@hms.harvard.edu
|
Phone |
617-919-2182
|
Organization name |
Boston Children's Hospital
|
Department |
Informatics Program
|
Street address |
320 Longwood Avenue
|
City |
Boston |
State/province |
MA |
ZIP/Postal code |
02115 |
Country |
USA |
|
|
Platform ID |
GPL96 |
Series (1) |
GSE26869 |
Regulation of myogenic progenitor proliferation in human fetal skeletal muscle by BMP4 and its antagonist Gremlin. |
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