|
| Status |
Public on Oct 10, 2023 |
| Title |
miR4734-t48_1 |
| Sample type |
RNA |
| |
|
| Source name |
miR4734-t48
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Human foreskin fibroblasts (HFF) (AG14608)
treatment: miR4734-t48
|
| Treatment protocol |
HFF cells were transfected either with miR-CTRL or miR-4734.
|
| Growth protocol |
Cells were grown in Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids (01-040-1A, Biological Industries, Israel) supplemented with 2.6 mM L-Glutamine, Ampicillin and Streptomycin and 15% FBS. For routine culturing, cell were split 1:3 or 1:4 every 3-4 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Qiazol (Qiagen, Germany)
|
| Label |
biotin
|
| Label protocol |
100 ng total RNA was taken for microarray analysis. Arrays were processed with Gene Chip WT PLUS reagent kit (REF 902281), according to Affymetrix standard protocols
|
| |
|
| Hybridization protocol |
According to Affymetrix standard protocols
|
| Scan protocol |
According to Affymetrix standard protocols, using Affymetrix GeneChip 3000 7G plus scanner
|
| Description |
Gene expression data
|
| Data processing |
RMA was carried out using Partek Genomic Suite 6.5 (Inc. St. Charles, MO; www.partek.com)
|
| |
|
| Submission date |
Oct 06, 2022 |
| Last update date |
Oct 10, 2023 |
| Contact name |
Ester Feldmesser |
| E-mail(s) |
ester.feldmesser@weizmann.ac.il
|
| Phone |
+972 8934 2614
|
| Organization name |
Weizmann Institute
|
| Department |
Life Science Core Facilities
|
| Street address |
POB 25
|
| City |
Rehovot |
| State/province |
Rehovot |
| ZIP/Postal code |
76100 |
| Country |
Israel |
| |
|
| Platform ID |
GPL23159 |
| Series (2) |
| GSE214962 |
A role for miR-4734 in conditional suppression of ER stress-associated pro-inflammatory responses [miR-CTRL or miR-4734-transfected HFF] |
| GSE214963 |
A role for miR-4734 in conditional suppression of ER stress-associated pro-inflammatory responses |
|
