|
| Status |
Public on Oct 12, 2022 |
| Title |
control_14 |
| Sample type |
genomic |
| |
|
| Source name |
peripheral white blood cells
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: peripheral white blood cells
diagnosis: control
Sex: male
|
| Growth protocol |
Peripheral venous blood was obtained after an overnight fast.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
After centrifugation, the plasma component of the blood was used for either immediate biochemistry analysis or for storage at -80o C for the subsequent measurements. Genomic DNA was then isolated from peripheral white blood cells using the phenol/chloroform DNA extraction method after lysis of red blood cells.
|
| Label |
biotin
|
| Label protocol |
Type 2 diabetes mellitus (DM) was defined as a fasting glucose larger than 126 mg/dL, a glycosylated hemoglobin larger than 6.5, or the use of hypoglycemic medications. CKD was defined using the National Kidney Foundation: Kidney Disease Outcomes Quality Initiative classification with a persistent proteinuria or an eGFR of less than 60 mL/min/1.73 m2, as determined by the abbreviated Modification of Diet in Renal Disease equation. DKD was diagnosed if subjects fulfilled both DM criteria and CKD criteria at the same time.
|
| |
|
| Hybridization protocol |
Using the Axiom Genome-Wide Array Plate technology platform provided by Thermo Fisher Scientific Inc.
|
| Scan protocol |
Using the Axiom Genome-Wide Array Plate technology platform provided by Thermo Fisher Scientific Inc.
|
| Description |
C0051220
|
| Data processing |
Import the CEL files into Axiom Analysis Suite, using Best Practices Workflow from the recommendations of the Axiom Genotyping Solution-Data Analysis Guide. Annotation and library downloaded from Taiwan Biobank (https://www.biobank.org.tw/fd.php)
processed data file includes: SNP ID: dbSNP_RS_ID and affy_snp_id; SNP Call: AA, AB, BB, and NoCall; SNP Annotation: Allele_A, Allele_B, Ref_Allele and Alt_Allele; SNP Quality: CR(Call Rate), ConversionType; SNP frequency: n_AA, n_AB, n_BB, n_NC and MinorAlleleFrequency.
|
| |
|
| Submission date |
Oct 11, 2022 |
| Last update date |
Oct 12, 2022 |
| Contact name |
Paul Wei-Che Hsu |
| E-mail(s) |
paul@nhri.edu.tw
|
| Phone |
+886-37-206-166
|
| Organization name |
National Health Research Institutes
|
| Department |
National Health Research Institutes
|
| Lab |
R2-3023
|
| Street address |
Zhunan: 35, Keyan Road, Zhunan Town
|
| City |
Miaoli County |
| State/province |
Taiwan |
| ZIP/Postal code |
350 |
| Country |
Taiwan |
| |
|
| Platform ID |
GPL32738 |
| Series (1) |
| GSE215221 |
Discovery of a Trans-Omics Biomarker Signature that Predisposes High Risk Diabetic Patients to Diabetic Kidney Disease |
|
