|
| Status |
Public on Mar 13, 2013 |
| Title |
NLS_Q13_dox+_2 |
| Sample type |
RNA |
| |
|
| Source name |
PC12 NLS-Q13 Dox(+), rep 2
|
| Organism |
Rattus norvegicus |
| Characteristics |
polyq: Q13
localization_signal: NLS
condition: dox+
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Inducible PC12 stable cells were cultured for 5 days with or without Dox and total cellular RNA were isolated by TRIzol (Invitrogen) according to the manufacture’s protocol.
|
| Label |
biotin
|
| Label protocol |
The samples were labeled using the Ambion WT expressio kit (Ambion) as described by the manufacturer.
|
| |
|
| Hybridization protocol |
Hybridization and signal detection of Rat Gene 1.0 ST Arrays (Affymetrix) was performed following the manufacturer's instruction using the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using Affymetrix GeneChip Scanner 3000.
|
| Description |
RNA was extracted from cells on day 5 after transfection.
|
| Data processing |
The data was normalized by robust multichip analysis (RMA) in affy library using R and Bioconductor.
|
| |
|
| Submission date |
Jan 28, 2011 |
| Last update date |
Mar 13, 2013 |
| Contact name |
Kaoru Mogushi |
| E-mail(s) |
mogushi-k@umin.ac.jp
|
| Phone |
+81-3-5802-1797
|
| Organization name |
Juntendo University
|
| Department |
Intractable Disease Research Center
|
| Street address |
2-1-1 Hongo
|
| City |
Bunkyo-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
113-8421 |
| Country |
Japan |
| |
|
| Platform ID |
GPL6247 |
| Series (1) |
| GSE26933 |
Effects of polyglutamine expansion and subcellular localization of C-terminal fragment of Cav2.1 in PC12 rat pheochromocytoma cells |
|
