|
| Status |
Public on Nov 29, 2011 |
| Title |
Ventricularis_Laminar_3_miRNA |
| Sample type |
RNA |
| |
|
| Source name |
Ventricularis HAVEC, Laminar Shear, 24h
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Aortic Valve, Endothelial Cells, Shear
group: Ventricularis_Laminar
|
| Treatment protocol |
fHAVEC and vHAVEC were sheared under oscillatory and laminar shear for 24h.
|
| Growth protocol |
Side-specific human aortic valvular endothelial cells (fHAVEC and vHAVEC) were grown in MCDB 131 supplemented with FBS, ECGS, IGF, EGF, FGF, VEGF, ascorbic acid, and hydrocortisone
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Qiazol and Qiagen's miRNeasy kit.
|
| Label |
biotin
|
| Label protocol |
Using suggested labelling protocol by Illumina.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol.
|
| Scan protocol |
Standard Illumina scanning protocol.
|
| Description |
replicate 3
|
| Data processing |
Because of low background, no normalization was used for data analysis. GenomeStudio V2010.1 was used to generate values.
|
| |
|
| Submission date |
Jan 28, 2011 |
| Last update date |
Nov 29, 2011 |
| Contact name |
Hanjoong Jo |
| E-mail(s) |
hanjoong.jo@bme.gatech.edu
|
| Phone |
404-712-9654
|
| Fax |
404-727-3330
|
| Organization name |
Emory University
|
| Department |
Biomedical Engineering
|
| Street address |
2005 WMB
|
| City |
Atlanta |
| State/province |
GA |
| ZIP/Postal code |
30068 |
| Country |
USA |
| |
|
| Platform ID |
GPL8179 |
| Series (1) |
| GSE26953 |
Discovery of Shear- and Side-specific mRNAs and miRNAs in Human Aortic Valvular Endothelial Cells |
|
