|
| Status |
Public on Jun 01, 2024 |
| Title |
input-effector-rep3 |
| Sample type |
SRA |
| |
|
| Source name |
Spleen
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Spleen
cell line: C57BL/6
cell type: effector CD8 (CD44+CD62L-CD8+)
genotype: WT
age: 6~8 weeks
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Wild-type naïve (CD44-CD62L+) CD8+ T cells, effector (CD44+CD62L-) CD8+ T cells and memory (CD44+CD62L+) CD8+ T cells were sorted. Total RNAs were extracted from indicated cells and then submitted to SLIM-Seq protocol.
SLIM-Seq data were analyzed as previously described (Rong Yin et al., 2022)
|
| |
|
| Library strategy |
RIP-Seq |
| Library source |
transcriptomic |
| Library selection |
other |
| Instrument model |
HiSeq X Ten |
| |
|
| Description |
in_EF3
|
| Data processing |
FastQC was used to identify features of the raw data that may indicate quality problems
Trimmomatic was used to trim raw fastq files
Kallisto was used to mapped to GRCm38 cDNA sequences
DESeq2 was used to calculate differential expression of m6A levels for each gene
Assembly: mm10
Supplementary files format and content: xls format. Read counts for each gene
|
| |
|
| Submission date |
Oct 14, 2022 |
| Last update date |
Jun 01, 2024 |
| Contact name |
Shuyang Yu |
| E-mail(s) |
ysy@cau.edu.cn
|
| Organization name |
China Agricultural University
|
| Department |
College of Biological Sciences
|
| Street address |
2 Yuanmingyuan West Road
|
| City |
Beijing |
| ZIP/Postal code |
100193 |
| Country |
China |
| |
|
| Platform ID |
GPL21273 |
| Series (2) |
| GSE215761 |
Mettl3-dependent m6A modification is essential for effector differentiation and memory formation of CD8+ T cells [SLIM-seq] |
| GSE215762 |
Mettl3-dependent m6A modification is essential for effector differentiation and memory formation of CD8+ T cells |
|
| Relations |
| BioSample |
SAMN31281061 |
| SRA |
SRX17889321 |
