|
| Status |
Public on Feb 08, 2011 |
| Title |
CCdl67TT_CCdl257TF_3d |
| Sample type |
RNA |
| |
|
| Source name |
Breast
|
| Organism |
Homo sapiens |
| Characteristics |
sample name: D21_04-18-06
tumor sample id: CCdl67TT
tumor sample histological grade: low, T1
fibroblast sample id: CCdl257TF
fibroblast sample histological grade: high, TF2
time: 3d
tissue: breat cancer tumor
treatment: tumor co-cultured with fibroblast
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total epithelial cell RNA was directly extracted from duplicate wells of each treatment using the Rneasy kit (Qiagen)
|
| Label |
biotin
|
| Label protocol |
cRNA synthesis was performed with 2 µg of total cellular RNA, and labelled based on the protocol outlined by Affymetrix Inc. (Santa Clara, CA), with few modifications
|
| |
|
| Hybridization protocol |
standard Affymetrix procedure
|
| Scan protocol |
standard Affymetrix procedure
|
| Data processing |
RMA using Affymetrix Expression Console
|
| |
|
| Submission date |
Feb 01, 2011 |
| Last update date |
Feb 08, 2011 |
| Contact name |
Junhee Seok |
| E-mail(s) |
jseok@stanford.edu
|
| Organization name |
Stanford University
|
| Street address |
318 Campus Drive W310 - Davis Lab
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE27018 |
Fibroblast triggered gene expression in tumor |
|
