|
| Status |
Public on Mar 17, 2023 |
| Title |
15XRi_ESC_MS235_002 |
| Sample type |
SRA |
| |
|
| Source name |
15XRi
|
| Organism |
Macaca fascicularis |
| Characteristics |
cell line: 15XRi
cell type: ESC
genotype: wildtype
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Total RNA was extracted by using RNeasy Mini Kit (QIAGEN, 74104) according to manufacturer’s instructions.
library was prepared by using NEBNext Ultra II Directional RNA Library Prep with Sample Purification Beads (NEB, E7765).
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
All read data were processed with cutadapt v1.18 and mapped with Tophat2 v2.1.1/bowtie2 v2.3.4.1 as described previously (Nakamura et.al.)
Expression levels as RPKM (Reads per kilobase of exon per million reads mapped) were calculated using cufflinks v2.2.1.
Assembly: macFas5.0
Supplementary files format and content: tab-delimited text files include FPKM for each Sample
|
| |
|
| Submission date |
Oct 20, 2022 |
| Last update date |
Mar 17, 2023 |
| Contact name |
Yukihiro Yabuta |
| E-mail(s) |
yabyab@anat2.med.kyoto-u.ac.jp
|
| Organization name |
Kyoto University, Graduate school of medicine
|
| Department |
Anatomy and Cell Biology
|
| Street address |
Yoshida-Konoe-cho, Sakyo-ku
|
| City |
Kyoto |
| State/province |
Kyoto |
| ZIP/Postal code |
606-8501 |
| Country |
Japan |
| |
|
| Platform ID |
GPL22523 |
| Series (2) |
| GSE216204 |
Induction of fetal meiotic oocytes from embryonic stem cells in cynomolgus monkeys ( RNA-Seq I ) |
| GSE216206 |
Induction of fetal meiotic oocytes from embryonic stem cells in cynomolgus monkeys |
|
| Relations |
| BioSample |
SAMN31389292 |
| SRA |
SRX17968895 |
