Growth in broth was achieved by transferring stock culture to MH broth in tissue culture flasks and incubated microaerobically. Growth on agar was achieved by transferring stock culture onto MH agar plates, spreading the culture to produce a lawn, and incubation microaerobically. Cell densities in both growth conditions increased steadily up to 12 hrs and maximum cell densities were achieved at 18 hrs following which cell death starts to occur. Thus based on the growth curve C. jejuni cells at 16 hrs of incubation were used in all studies.
