|
| Status |
Public on Feb 28, 2011 |
| Title |
blood-aCGH-patient 29 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
Peripheral blood
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Male
test: patient 29
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted from lymphoblastoid cell lines, peripheral blood, or cultured tissues of the subjects and phenotypically normal male and female references using an M48 or the Puregene DNA isolation kit according to the manufacturer's instructions.
|
| Label |
Cy5
|
| Label protocol |
500 ng of sonicated DNA was labeled with Cy5 or Cy3 using BioPrime Total labeling kit (Invitrogen) per manufacturer's instructions
|
| |
|
| Channel 2 |
| Source name |
Peripheral blood
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Male
reference: control
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted from lymphoblastoid cell lines, peripheral blood, or cultured tissues of the subjects and phenotypically normal male and female references using an M48 or the Puregene DNA isolation kit according to the manufacturer's instructions.
|
| Label |
Cy3
|
| Label protocol |
500 ng of sonicated DNA was labeled with Cy5 or Cy3 using BioPrime Total labeling kit (Invitrogen) per manufacturer's instructions
|
| |
|
| |
| Hybridization protocol |
Hybridization and washing were perfomed with Agilent reagents per manufacturer's instructions
|
| Scan protocol |
Scanned on Axon 4000B scanner (Molecular Devices)
|
| Data processing |
Agilent Feature Extraction software v9.5.3.1 and Agilent CGH Analytics software v3.5.14 and displayed using oligonucleotide aCGH analysis software Genoglyphix (Signature Genomics).
The standard Agilent protocol CGH-v4_95_Feb07 was used to normalize the data obtained on this platform.
|
| |
|
| Submission date |
Feb 04, 2011 |
| Last update date |
Feb 28, 2011 |
| Contact name |
Blake C. Ballif |
| Organization name |
Signature Genomic Laboratories
|
| Department |
Research & Development
|
| Street address |
2820 North Astor Street
|
| City |
Spokane |
| State/province |
WA |
| ZIP/Postal code |
99207 |
| Country |
USA |
| |
|
| Platform ID |
GPL10004 |
| Series (1) |
| GSE27080 |
Detection of unbalanced insertions by aCGH and FISH |
|
