|
| Status |
Public on Nov 03, 2022 |
| Title |
MF_Gm_csfKO_pnd9_2 |
| Sample type |
RNA |
| |
|
| Source name |
Lung
|
| Organism |
Mus |
| Characteristics |
strain: C57BL/6
metadata info: metaData_microarrays.txt
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were sorted into 500ul RLT buffer (QIAGEN). RNA was isolated using the RNeasy micro kit (QIAGEN) and sent to the Nucleomics facility, VIB Leuven, Belgium where the microarrays were performed using the GeneChip Mouse Gene 1.0 ST arrays (Affymetrix).
|
| Label |
biotin
|
| Label protocol |
standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
standard Affymetrix protocol
|
| Scan protocol |
Affymetrix Gene ChIP Scanner 3000 7G
|
| Description |
GM-CSF KO macrophages on post natal day 9
GM-CSF KO macrophages on post natal day 9 treated with PBS
|
| Data processing |
Samples were analyzed using R/limma: Robust Multi-array Average (RMA) procedure was used to normalize data within arrays (probeset summarization, background correction and log2-transformation) and between arrays (quantile normalization). Only probesets that uniquely mapped to an EntrezID were kept.
|
| |
|
| Submission date |
Oct 27, 2022 |
| Last update date |
Nov 03, 2022 |
| Contact name |
Liesbet Martens |
| E-mail(s) |
liesbet.martens@irc.vib-ugent.be
|
| Organization name |
VIB-University of Ghent
|
| Department |
VIB Inflammation Research Center
|
| Street address |
Technologiepark 927
|
| City |
Zwijnaarde |
| ZIP/Postal code |
9052 |
| Country |
Belgium |
| |
|
| Platform ID |
GPL6246 |
| Series (2) |
| GSE216701 |
Loss of evolutionary conserved GM-CSF-dependent signature in pulmonary macrophages in COVID-19 provides a rationale for inhaled GM-CSF treatment (microarray) |
| GSE216705 |
Loss of GM-CSF-dependent instruction of alveolar macrophages in COVID-19 provides a rationale for inhaled GM-CSF treatment |
|
