|
| Status |
Public on Nov 01, 2022 |
| Title |
Recipient cow 3 |
| Sample type |
SRA |
| |
|
| Source name |
uterine flushing fluids
|
| Organism |
Bos taurus |
| Characteristics |
tissue: uterine flushing fluids
breed: Xianan
rna popoulation: exosomal miRNA
|
| Treatment protocol |
On the 7th day after artificial insemination of donors, 100 ml bovine UFs were collected by uterine flushing using sterile Gibco Minimum Essential Media with 0.7% 200mM Tris buffer (pH = 7.2) controls.Three donor cows with well-developed blastocysts were used for further analysis . Three recipient cows having similar body weight and age were selected as matched.
|
| Growth protocol |
Ten Xianan cows were selected as donors for synchronous estrus and superovulation. The artificial insemination was performed after superovulation. Another 23 Xianan cows were selected as recipients for synchronous estrus.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA in exosomes from uterine flushing fluids was extracted using the miRNeasy Serum/Plasma Kit (Qiagen, Germany)
A total of 10 ng total RNA was used to construct the small RNA library using the TruSeq Small RNA Sample Prep Kits (Illumina, USA).
|
| |
|
| Library strategy |
miRNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
HiSeq X Ten |
| |
|
| Data processing |
Agilent Technologies 2100 Bioanalyze was used to check the size and purity of the library.
Clean reads were mapped to the bovine genome (ARS-UCD 1.2) using HISAT2
Htseq package was used to get gene counts
Assembly: ARS-UCD 1.2
Supplementary files format and content: tab-delimited text files include RPKM values for each Sample
|
| |
|
| Submission date |
Oct 27, 2022 |
| Last update date |
Nov 01, 2022 |
| Contact name |
Shijie Lyu |
| Organization name |
Henan Academy of Agricultural Sciences
|
| Street address |
Huayuan Road 116
|
| City |
Zhengzhou |
| ZIP/Postal code |
450002 |
| Country |
China |
| |
|
| Platform ID |
GPL24230 |
| Series (1) |
| GSE216746 |
RNA-seq analysis of uterine flushing fluids of three donor and recipient Xianan cows 7 days after fertilization |
|
| Relations |
| BioSample |
SAMN31493611 |
| SRA |
SRX18050884 |
