|
| Status |
Public on Nov 15, 2023 |
| Title |
sgNTC_2_day7 |
| Sample type |
RNA |
| |
|
| Source name |
sgNTC OT-I TILs isolated from B16-OVA tumors 7 days after adoptive transfer
|
| Organism |
Mus musculus |
| Characteristics |
cell type: OT-I TILs isolated from B16-OVA tumors 7 days after adoptive transfer
transduced with: sgNTC
|
| Treatment protocol |
Sorted cells were lysed with lysis buffer from Rneasy Micro kit from Qiagen.
|
| Growth protocol |
Cells were sort-purified in the sgRNA transduced OT-I cells from B16-OVA tumors 7 days after adoptive transfer
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA samples were prepared according to the prototol of Rneasy Micro kit from Qiagen,
|
| Label |
biotin
|
| Label protocol |
Total RNA (1 - 20 ng) were converted into biotinylated cDNA using the NuGEN WTA Pico v2 protocol and the NuGEN Encore Biotin module
|
| |
|
| Hybridization protocol |
Six micrograms of biotinylated cDNA was fragmented and then hybridized for 16 hr at 45C on an Affymetrix Mouse Gene 2.0 ST Array. Following hybridization, GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the AffymetrixGeneChip Scanner 3000 7G.
|
| Data processing |
Probe signals were quantile normalized and summarized by the RMA algorithm using the Affymetrix Expression Console software v1.1
|
| |
|
| Submission date |
Oct 27, 2022 |
| Last update date |
Nov 15, 2023 |
| Contact name |
Hongbo Chi |
| E-mail(s) |
hongbo.chi@stjude.org
|
| Organization name |
St Jude Children's Research Hospital
|
| Department |
Immunology
|
| Street address |
262 Danny Thomas Place
|
| City |
Memphis |
| State/province |
TN |
| ZIP/Postal code |
38105 |
| Country |
USA |
| |
|
| Platform ID |
GPL23038 |
| Series (2) |
| GSE216749 |
Single-cell CRISPR screens in vivo map T cell fate regulomes in cancer [microarray] |
| GSE216800 |
Single-cell CRISPR screens in vivo map T cell fate regulomes in cancer |
|
